基于2D-DIGE/MALDI-TOF-MS技术筛选奶牛低血钙症生物标志物

doi:10.11843/j.issn.0366-6964.2015.07.022

Abstract

Abstract:

Milk fever (MF) is a seriously nutrition metabolism disease which is divide into clinical and subclinical hypocalcemia (SH).The level of Ca in blood combining the clinical signal were always used to diagnose MF.But in the total development progress of this disease the pathogenesis was not clearly.In this study we screened the biomarkers of MF and SH，and want to reveal the possibly pathogenesis.Twenty-seven dairy cows were selected as the experiment animal，and there were 3 groups MF，SH and C according to the blood calcium concentration and the clinical signal of these dairy cows.Every 3 samples were mixed into a hybrid sample in each group，and 9 hybrid samples were crossed into the 2D-DIGE.Eighty of 110 spots，which were found via 2D-DIGE，were selected into mass spectrum.Sixty-six spots were found，and were identified into 16 proteins.A2M，HP，PON1 were selected in verification test by using Western blotting，and the results support the results of DIGE experiment.In this study 16 proteins were found among three groups，and 3 proteins of them were verified first time.Collectively，the results indicate that A2M，HP，PON1 are 3 novel biomarkers for MF，and they may contribute to explore the possible mechanism of MF.

CLC Number:

S857.2

SHU Shi，XIA Cheng，ZHANG Hong-you，XU Chu-chu，LI Chang-sheng，XIAO Xin-huan，WANG Gang，BAI Yun-long. Biomarker Discovery for Cows with Hypocalcemia Using 2D-DIGE[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1238-1245.

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Biomarker Discovery for Cows with Hypocalcemia Using 2D-DIGE

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